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KMID : 0861020120270040009
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Korea Journal of Herbology
2012 Volume.27 No. 4 p.9 ~ p.16
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Enhancing effect of Canavalia gladiata DC semen on the hematopoietic expansion and function of stem cells
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Kim Seung-Hyung
Kim Kun-Hoae
Jee Kyung-Yup
Cho In-Sik
Kim Han-Young
Lee Young-Cheol
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Abstract
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Objective: Canavalia gladiata DC semen (CGS) have been used to improve hematopoietic activity. In the current study, we investigated whether CGS regulate hemato-potentiating function using hematopoietic stem cells (HSCs) as a testing system.
Methods: HSCs isolated from femur in mice with leukopenia and thrombocytopenia induced induced by CTX. Then, Real-time PCR was performed to measure the mRNA expression and hematopoietic related gene (EPO, IL-3, SCF, c-kit, GM-CSF), the phoaphorylation of GATA-1 and STAT-5a/b were observed by ELISA method, and the number of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E), semisolid clonogenic assay was performed.
Result: When HSCs were treated with CGS, the expression of hematopoietic related genes (EPO, IL-3, SCF, c-kit, and GM-CSF) were significantly increased at the levels of mRNA as well as production in HSCs. Additionally, CGS enhanced phosphorylation of STAT-1 and signal transducer and activator of transcription-5a/b (STAT-5a/b) in HSCs. Furthermore, CGS significantly enhanced the growth rate of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E) in vitro.
Conclusion: These result suggest that CGS has hematopoietic enhancement via hematopoietic cytokine-mediated GATA-1/STAT-5a/b pathway.
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KEYWORD
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Hematopoietic, Canavalia gladiata DC semen, Cyclophosphamide, GATA-1, STAT-5a, b
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